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Recombinant proteins were purified with glutathione Sepharose resin (GE Healthcare) as previously described (Berton kinase assays were performed by incubating cyclin D3\CDK6 or cyclin D1\CDK4 active kinase complexes (Signal Chem) with GST\tagged FOXO3a full length (Abnova), GST\FOXO3 mutants, or GST\RB1 recombinant proteins, as substrates, for 30?min at 30C in the presence of 1
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Recombinant proteins were purified with glutathione Sepharose resin (GE Healthcare) as previously described (Berton kinase assays were performed by incubating cyclin D3\CDK6 or cyclin D1\CDK4 active kinase complexes (Signal Chem) with GST\tagged FOXO3a full length (Abnova), GST\FOXO3 mutants, or GST\RB1 recombinant proteins, as substrates, for 30?min at 30C in the presence of 1

cmerp January 3, 2023
Recombinant proteins were purified with glutathione Sepharose resin (GE Healthcare) as previously described (Berton kinase assays were...
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