Our data confirmed that, simultaneous reactivity with anti-dsDNA, -nucleosome and -histone antibodies (3-pos) in patients with SLE were highly relevant to LN pathology. examined by pathologists. Immune complex deposition CM-675 was identified by immunohistochemistry stain. Results Simultaneous positivity of anti-dsDNA, -nucleosome and -histone antibodies (3-pos) was prevalent in SLE patients with LN compared to Non-renal SLE patients (41% vs 11%, report that serum anti-C1q antibody level is positively associated with glomerular C1q deposition in LN [5]. Prevalence of anti-C1q, anti-dsDNA and anti-chromatin/nucleosome antibodies in Juvenile SLE (JSLE) patients is positively associated with LN and disease activity. Furthermore, these antibodies are sensitive and specific for diagnosis of JSLE [6]. Meanwhile, serum anti- actin antibody seems to be a reliable biomarker for renal involvement in SLE patients, yet relevant antibody is not found in renal biopsy [7, 8]. We previously report that, in SLE patients, simultaneously positivity for anti-dsDNA, anti-nucleosome and anti-histone antibodies by Euroline ANA Profile (IgG) test is significantly relevant with LN onset and activity, and suggestive as a valuable indicator for renal involvement [9]. On the other side, many results demonstrate that anti-dsDNA antibody and other immune related components such as the levels of C3, C4 or anti-nucleosome antibody are negatively related with LN progression [10C14]. There are still no specific biomarkers that are publicly accepted for indicting SLE or LN pathology due to the variation of SLE population, region and measurements. In this study, we collected and analyzed data of more than a decade (2002 to 2013) of SLE patients from Heilongjiang province, the northeast region of China, with a population of more than 38 millions. CM-675 The region has a typical climate in the frigid-temperate zone and goes through three to four months frost-free period each year, which may relate to local Rabbit Polyclonal to CEBPG morbidity of SLE and rheumatoid arthritis [15C17]. Our data confirmed that, simultaneous reactivity with anti-dsDNA, -nucleosome and -histone antibodies (3-pos) in patients with SLE were highly relevant to LN pathology. Three-pos LN patients showed significantly higher serum levels of these antibodies, suffered from more severe renal damage and needed more intensive treatments than non-3-pos LN patients, indicating 3-pos as an indicative biomarker for severe LN. Patients and Methods Ethical considerations All participants provided written consent for study participation. This consent procedure and the study were reviewed and approved by the National Ethical Committee of the Public Health School of the Harbin Medical University, in compliance with the principles of the Helsinki Declaration II. Patient samples All study participants attended SLE clinic at the 1st and 2nd Hospital Affiliated to Harbin Medical University from 2002 to 2013. All the patients with SLE met the American College of Rheumatology (ACR) classification criteria for SLE. 921 LN patients (854 females, 67 males, median age 35 years, range 9C80 years) and 778 patients without nephritis (724 females, 54 males, median age 34 years, range 10C80 years) were enrolled (S1 Table). LN patients were classified using microscopic analysis of urinary sediments, 24 hour proteinuria, serum creatinine and complement C3 levels, in which 211 (23%) of LN patients (195 females, 16 males, age 35.0924.38) were confirmed using renal biopsies as per the International Society of Nephrology (ISN) and WHO criteria for SLE nephritis. Peripheral blood serum (with 42 paired serum) was taken at diagnosis and remission (partial/complete) for measurement of serum autoantibodies, renal parameters including urinary sediment assessment, 24h urinary protein, Scr and blood urea nitrogen (BUN). Levels of C3 and C4 were determined by an automatic analyzer. Disease activity was assessed by systemic lupus erythematosus disease activity index (SLEDAI). Assays of anti-nuclear antibodies profile ANA profile (mitochondrial-2, CM-675 ribosomal-p, histone, nucleosome, dsDNA, PCNA, centromere, Jo-1, PM-Scl, Scl-70, SSB, Ro-52, SSA, sm, RNP) was detected by EUROLINE ANA profile (IgG) kit according to manufacturers instruction CM-675 (EUROLINE, Lbeck, Germany). The results were read.