Sera were tested at a 1:100 dilution using the DENV IgG Dx Select and DENV IgM Capture Dx Select packages (Focus Diagnostics Inc

Sera were tested at a 1:100 dilution using the DENV IgG Dx Select and DENV IgM Capture Dx Select packages (Focus Diagnostics Inc., Cypress, CA, USA) according to the manufacturers instructions. activity in Bangladesh, a prospective cohort study involving serological surveys was undertaken with participant interviews and blood donation across the city of Dhaka in 2012. Study participants were recruited from 12 of 90 wards and blood samples were collected during both the pre-monsoon (n = 1125) and post-monsoon (n = 600) seasons of 2012. The findings revealed that this seroprevalence in all pre-monsoon samples was 80.0% (900/1125) while RGH-5526 the seropositivity in the pre-monsoon samples that experienced paired post-monsoon samples was 83.3% (503/600). Of the 97 paired RGH-5526 samples that were unfavorable at the pre-monsoon time point, 56 were positive at the post-monsoon time point. This resulted in a seroprevalence of 93.2% (559/600) among individuals tested during the post-monsoon period. Seroprevalence trended higher with age with children exhibiting a lower seropositivity as compared to adults. Results from this study also indicated that DENV strains were the only flaviviruses circulating in Dhaka in 2012. A multivariate analysis revealed that age, possession of interior potted plants, and types of mosquito control steps were significant factors associated with DENV seroprevalence; while attendance in public/mass gatherings, and use of mosquito control steps were significantly associated with DENV seroconversion after adjusting for all other variables. Conclusions/Significance Our study suggests that there is RGH-5526 a high level of endemic dengue computer virus circulation in the city of Dhaka which has resulted in significant DENV seroprevalence among its residents. Seropositivity increased with age, however, a substantial proportion of children are at risk for DENV infections. Our serological analysis also documents considerable DENV seroconversion among study participants which indicates that a large proportion of the population in the city of Dhaka were newly exposed to DENV during the study period (pre-and post-monsoon 2012). High levels of seroconversion suggest that there was an intense blood circulation of DENV in 2012 and this may have resulted in a significant risk for viral associated illness. Findings of our study further indicated that home-based interventions, such as removing interior potted plants and increased bed net use, in addition to vector control steps in public parks, would reduce exposure to DENV and further decrease risk of viral associated disease. Author summary Comparable to many other tropical regions of the world, dengue is a major public health problem in Bangladesh where mosquitoes are the main vector. Through this serological survey, we present data around the magnitude (measured in proportions) of seroprevalence and seroconversion within 12 selected wards in the city of Dhaka, Bangladesh. In 2012 the observed dengue seroprevalence was 93% among individuals tested during post-monsoon with a seroconversion rate between pre- and post-monsoon periods of 57.7%. This obtaining suggests RGH-5526 that dengue computer virus (DENV) circulated in the city during the observation period. Recent exposure to dengue computer virus was highly associated with age, possession of interior potted plants, types of mosquito control steps used, and human movement and attendance in mass gatherings, while the extent of dengue seroprevalence were not associated with the socioeconomic status of the study participants. Our findings suggest that household Rabbit Polyclonal to MRPL16 utilities and water management or storage practices and acknowledgement and removal of mosquito development sites and participation in mass gatherings are important factors that impact exposure to dengue. Intervention strategies should therefore target these factors for effective prevention and control of dengue contamination. Introduction The spread of dengue computer virus (DENV)Ca viral pathogen transmitted by mosquitoes, primarily and was defined as the percentage of total individuals in a populace who tested positive for the presence of IgG antibodies to DENV. Individuals identified as were found to be unfavorable for dengue antibody during the pre-monsoon period but who became positive for dengue IgM or IgG based on the serological analysis of their postCmonsoon sample. The extent of seroconversion refers to the percentage of new cases as well as new exposures identified between the pre- and post-monsoon period. Two household serosurveys were conducted in 2012 in order to assess the seroprevalence and seroincidence of DENV. The first serosurvey was completed during the pre-monsoon season of 2012 (June and July). At this time, information on demographic, socio-economic and other relevant characteristics was collected from the study participants using a structured socioeconomic questionnaire. The inclusion criteria for household members in our study were: all household members shared one roof, all meals, and common living space..